Pieńkowska J.R., Proćków M., Lesicki A.

pienkowj@amu.edu.pl

On the basis of integrative analysis of molecular (gene sequences) and morphological (shell and genitalia) features Pieńkowska et al. (2018, 2019) recognised six distinct lineages of Monacha cantiana s.l.: CAN-1 – CAN-6. M. cantiana CAN-1 which occurs in the United Kingdom, Spain and the Latium region in Italy was assigned to M. cantiana s.str. Lineage M. cantiana CAN-2 occurs in northern Italy (Venetum and Lombardy), CAN-3 in northern Italy (Emilia Romagna and Friuli-Venezia Giulia) and also in Austria (Waldviertel and Vienna), CAN-4 (recognised as M. cemenelea) in southern France (Sainte Thecle near Nice). The lineages CAN-5 and CAN-6 with narrow distributions in Apuan Alps (Italy) represent different species, however selection of their names requires further studies.

We continue the analysis of distribution of M. cantiana in Europe using molecular markers (sequences of selected mitochondrial and nuclear genes). DNA obtained from foot tissue of specimens collected in ten localities in northern France and two in the Netherlands was a template for standard PCR amplification for 5’end of mitochondrial COI gene (barcode sequence). Neighbour Joining and Maximum Likelihood analyses of 597 bp long COI haplotypes were performed with MEGA7. Four COI haplotypes: COI 1 – COI 4 were identified among 44 new sequences. Haplotype CAN-1 was common in specimens from all studied populations. On the contrary, haplotypes COI 2, 3 and 4 were rare, found in single specimens in one French and two Dutch populations. Sequences of these four haplotypes were compared with sequences representing all M. cantiana mitochondrial lineages and some other Monacha species. Haplotypes COI 1 – COI 4 clustered in ML/NJ tree with sequences of M. cantiana CAN-1. Molecularly, it proves that M. cantiana s.str. occurs in northern France and the Netherlands, however further analyses of nuclear genes as well as the structure of shells and reproductive tract are in progress.

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